RT Journal Article SR Electronic T1 The WPRE improves genetic engineering with site-specific nucleases JF bioRxiv FD Cold Spring Harbor Laboratory SP 126904 DO 10.1101/126904 A1 Jessica M. Ong A1 Christopher R. Brown A1 Matthew C. Mendel A1 Gregory J. Cost YR 2017 UL http://biorxiv.org/content/early/2017/04/12/126904.abstract AB Abstract Inclusion of the woodchuck hepatitis virus post-transcriptional response element (WPRE) in the 3’ UTR of mRNA encoding zinc-finger or TALE nucleases results in up to a fifty-fold increase in nuclease expression and a several-fold increase in nuclease-modified chromosomes. Significantly, this increase is additive with the enhancement generated by transient hypothermic shock. The WPRE-mediated improvement is seen across several types of human and mouse primary and transformed cells and is translatable in vivo to the mouse liver.