TY - JOUR T1 - The WPRE improves genetic engineering with site-specific nucleases JF - bioRxiv DO - 10.1101/126904 SP - 126904 AU - Jessica M. Ong AU - Christopher R. Brown AU - Matthew C. Mendel AU - Gregory J. Cost Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/04/12/126904.abstract N2 - Abstract Inclusion of the woodchuck hepatitis virus post-transcriptional response element (WPRE) in the 3’ UTR of mRNA encoding zinc-finger or TALE nucleases results in up to a fifty-fold increase in nuclease expression and a several-fold increase in nuclease-modified chromosomes. Significantly, this increase is additive with the enhancement generated by transient hypothermic shock. The WPRE-mediated improvement is seen across several types of human and mouse primary and transformed cells and is translatable in vivo to the mouse liver. ER -