@article {Ong126904, author = {Jessica M. Ong and Christopher R. Brown and Matthew C. Mendel and Gregory J. Cost}, title = {The WPRE improves genetic engineering with site-specific nucleases}, elocation-id = {126904}, year = {2017}, doi = {10.1101/126904}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Abstract Inclusion of the woodchuck hepatitis virus post-transcriptional response element (WPRE) in the 3{\textquoteright} UTR of mRNA encoding zinc-finger or TALE nucleases results in up to a fifty-fold increase in nuclease expression and a several-fold increase in nuclease-modified chromosomes. Significantly, this increase is additive with the enhancement generated by transient hypothermic shock. The WPRE-mediated improvement is seen across several types of human and mouse primary and transformed cells and is translatable in vivo to the mouse liver.}, URL = {https://www.biorxiv.org/content/early/2017/04/12/126904}, eprint = {https://www.biorxiv.org/content/early/2017/04/12/126904.full.pdf}, journal = {bioRxiv} }