PT  - JOURNAL ARTICLE
AU  - Rupam Jha
AU  - Johanna Roostalu
AU  - Martina Trokter
AU  - Thomas Surrey
TI  - Combinatorial regulation of the balance between dynein microtubule end accumulation and initiation of directed motility
AID  - 10.1101/126508
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 126508
4099  - http://biorxiv.org/content/early/2017/04/11/126508.short
4100  - http://biorxiv.org/content/early/2017/04/11/126508.full
AB  - Cytoplasmic dynein is involved in a multitude of essential cellular functions. Dynein’s activity is controlled by the combinatorial action of several regulators. The molecular mechanism of this regulation is poorly understood. Using purified proteins, we reconstitute the regulation of the human dynein complex by three prominent regulators on dynamic microtubules in the presence of end binding proteins (EBs). We find that dynein can be in biochemically and functionally distinct pools: either passively tracking dynamic microtubule plus-ends in an EB-dependent manner or moving processively towards minus ends in an adaptor protein-dependent manner. Whereas both dynein pools share the dynactin complex, they have opposite preferences for binding other regulators, either the adaptor protein Bicaudal D2 (BicD2) or the multifunctional regulator Lisencephaly-1 (Lis1). Remarkably, dynactin, but not EBs, strongly biases motility initiation locally from microtubule plus ends by autonomous plus end recognition. BicD2 and Lis1 together control the overall efficiency of motility initiation. Our study provides insight into the mechanism of dynein activity regulation by dissecting the distinct functional contributions of the individual members of a dynein regulatory network.