RT Journal Article
SR Electronic
T1 A versatile compressed sensing scheme for faster and less phototoxic 3D fluorescence microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 125815
DO 10.1101/125815
A1 Maxime Woringer
A1 Xavier Darzacq
A1 Christophe Zimmer
A1 Mustafa Mir
YR 2017
UL http://biorxiv.org/content/early/2017/04/10/125815.abstract
AB Three-dimensional fluorescence microscopy based on Nyquist sampling of focal planes faces harsh trade-offs between acquisition time, light exposure, and signal-to-noise. We propose a 3D compressed sensing approach that uses temporal modulation of the excitation intensity during axial stage sweeping and can be adapted to fluorescence microscopes without hardware modification. We describe implementations on a lattice light sheet microscope and an epifluorescence microscope, and show that images of beads and biological samples can be reconstructed with a 5-10 fold reduction of light exposure and acquisition time. Our scheme opens a new door towards faster and less damaging 3D fluorescence microscopy.OCIS codes: (110.1758) Computational imaging; (170.2520) Fluorescence microscopy; (170.6900) Three-dimensional microscopy.