RT Journal Article
SR Electronic
T1 Establishment in Culture of Expanded Potential Stem Cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 124479
DO 10.1101/124479
A1 Jian Yang
A1 David J. Ryan
A1 Wei Wang
A1 Jason Cheuk-Ho Tsang
A1 Guocheng Lan
A1 Hideki Masaki
A1 Xuefei Gao
A1 Liliana Antunes
A1 Yong Yu
A1 Zhexin Zhu
A1 Juexuan Wang
A1 Aleksandra A. Kolodziejczyk
A1 Lia S. Campos
A1 Cui Wang
A1 Fengtang Yang
A1 Zhen Zhong
A1 Beiyuan Fu
A1 Melanie Eckersley-Maslin
A1 Michael Woods
A1 Yosuke Tanaka
A1 Adam C. Wilkinson
A1 James Bussell
A1 Jacqui White
A1 Ramiro Ramirez-Solis
A1 Wolf Reik
A1 Berthold Göttgens
A1 Sarah A. Teichmann
A1 Hiromitsu Nakauchi
A1 Xiangang Zou
A1 Liming Lu
A1 Pentao Liu
YR 2017
UL http://biorxiv.org/content/early/2017/04/06/124479.abstract
AB Mouse embryonic stem cells are derived from in vitro explantation of blastocyst epiblasts1,2 and contribute to both the somatic lineage and germline when returned to the blastocyst3 but are normally excluded from the trophoblast lineage and primitive endoderm4–6. Here, we report that cultures of expanded potential stem cells (EPSCs) can be established from individual blastomeres, by direct conversion of mouse embryonic stem cells (ESCs) and by genetically reprogramming somatic cells. Remarkably, a single EPSC contributes to the embryo proper and placenta trophoblasts in chimeras. Critically, culturing EPSCs in a trophoblast stem cell (TSC) culture condition permits direct establishment of TSC lines without genetic modification. Molecular analyses including single cell RNA-seq reveal that EPSCs share cardinal pluripotency features with ESCs but have an enriched blastomere transcriptomic signature and a dynamic DNA methylome. These proof-of-concept results open up the possibility of establishing cultures of similar stem cells in other mammalian species.