TY - JOUR T1 - Establishment in Culture of Expanded Potential Stem Cells JF - bioRxiv DO - 10.1101/124479 SP - 124479 AU - Jian Yang AU - David J. Ryan AU - Wei Wang AU - Jason Cheuk-Ho Tsang AU - Guocheng Lan AU - Hideki Masaki AU - Xuefei Gao AU - Liliana Antunes AU - Yong Yu AU - Zhexin Zhu AU - Juexuan Wang AU - Aleksandra A. Kolodziejczyk AU - Lia S. Campos AU - Cui Wang AU - Fengtang Yang AU - Zhen Zhong AU - Beiyuan Fu AU - Melanie Eckersley-Maslin AU - Michael Woods AU - Yosuke Tanaka AU - Adam C. Wilkinson AU - James Bussell AU - Jacqui White AU - Ramiro Ramirez-Solis AU - Wolf Reik AU - Berthold Göttgens AU - Sarah A. Teichmann AU - Hiromitsu Nakauchi AU - Xiangang Zou AU - Liming Lu AU - Pentao Liu Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/04/06/124479.abstract N2 - Mouse embryonic stem cells are derived from in vitro explantation of blastocyst epiblasts1,2 and contribute to both the somatic lineage and germline when returned to the blastocyst3 but are normally excluded from the trophoblast lineage and primitive endoderm4–6. Here, we report that cultures of expanded potential stem cells (EPSCs) can be established from individual blastomeres, by direct conversion of mouse embryonic stem cells (ESCs) and by genetically reprogramming somatic cells. Remarkably, a single EPSC contributes to the embryo proper and placenta trophoblasts in chimeras. Critically, culturing EPSCs in a trophoblast stem cell (TSC) culture condition permits direct establishment of TSC lines without genetic modification. Molecular analyses including single cell RNA-seq reveal that EPSCs share cardinal pluripotency features with ESCs but have an enriched blastomere transcriptomic signature and a dynamic DNA methylome. These proof-of-concept results open up the possibility of establishing cultures of similar stem cells in other mammalian species. ER -