@article {Yang124479, author = {Jian Yang and David J. Ryan and Wei Wang and Jason Cheuk-Ho Tsang and Guocheng Lan and Hideki Masaki and Xuefei Gao and Liliana Antunes and Yong Yu and Zhexin Zhu and Juexuan Wang and Aleksandra A. Kolodziejczyk and Lia S. Campos and Cui Wang and Fengtang Yang and Zhen Zhong and Beiyuan Fu and Melanie Eckersley-Maslin and Michael Woods and Yosuke Tanaka and Adam C. Wilkinson and James Bussell and Jacqui White and Ramiro Ramirez-Solis and Wolf Reik and Berthold G{\"o}ttgens and Sarah A. Teichmann and Hiromitsu Nakauchi and Xiangang Zou and Liming Lu and Pentao Liu}, title = {Establishment in Culture of Expanded Potential Stem Cells}, elocation-id = {124479}, year = {2017}, doi = {10.1101/124479}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Mouse embryonic stem cells are derived from in vitro explantation of blastocyst epiblasts1,2 and contribute to both the somatic lineage and germline when returned to the blastocyst3 but are normally excluded from the trophoblast lineage and primitive endoderm4{\textendash}6. Here, we report that cultures of expanded potential stem cells (EPSCs) can be established from individual blastomeres, by direct conversion of mouse embryonic stem cells (ESCs) and by genetically reprogramming somatic cells. Remarkably, a single EPSC contributes to the embryo proper and placenta trophoblasts in chimeras. Critically, culturing EPSCs in a trophoblast stem cell (TSC) culture condition permits direct establishment of TSC lines without genetic modification. Molecular analyses including single cell RNA-seq reveal that EPSCs share cardinal pluripotency features with ESCs but have an enriched blastomere transcriptomic signature and a dynamic DNA methylome. These proof-of-concept results open up the possibility of establishing cultures of similar stem cells in other mammalian species.}, URL = {https://www.biorxiv.org/content/early/2017/04/06/124479}, eprint = {https://www.biorxiv.org/content/early/2017/04/06/124479.full.pdf}, journal = {bioRxiv} }