RT Journal Article
SR Electronic
T1 Cross-talk between active DNA demethylation, resetting of cellular metabolism and shoot apical growth in poplar bud break
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 122119
DO 10.1101/122119
A1 Daniel Conde
A1 Mariano Perales
A1 Anne-Laure Le Gac
A1 Christopher Dervinis
A1 Matias Kirst
A1 Stéphane Maury
A1 Pablo González-Melendi
A1 Isabel Allona
YR 2017
UL http://biorxiv.org/content/early/2017/04/03/122119.abstract
AB Annual dormancy-growth cycle is a developmental and physiological process essential for the survival of temperate and boreal forests. Seasonal control of shoot growth in woody perennials requires specific genetic programs integrated with the environmental signals. The environmental-controlled mechanisms that regulate the shift between winter dormancy to growth promoting genetic program are still unknown. Here, we show that dynamics in genomic DNA methylation (gDNA) levels regulate dormancy-growth cycle in poplar. We proved that the reactivation of cell division in the apical shoot that lead bud break process in spring, is preceded by a progressive reduction of gDNA methylation in apex tissue. We also identified that the induction in apex tissue of a chilling-dependent poplar DEMETER-LIKE 10 (PtaDML10) DNA demethylase precedes shoot growth reactivation. Transgenic poplars showing down-regulation of PtaDML8/10 caused delayed bud break. Genome wide transcriptome and methylome analysis and data mining revealed the gene targets of active DML-dependent DNA demethylation genetically associated to bud break. These data point to a chilling dependent-DEMETER-like DNA demethylase controlling the genetic shift from winter dormancy to a condition that promotes shoot apical vegetative growth in poplar.