RT Journal Article
SR Electronic
T1 Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 122382
DO 10.1101/122382
A1 Katarzyna Jezierska-Wozniak
A1 Seweryn Lipiński
A1 Łukasz Grabarczyk
A1 Monika Barczewska
A1 Aleksandra Habich
A1 Joanna Wojtkiewicz
A1 Wojciech Maksymowicz
YR 2017
UL http://biorxiv.org/content/early/2017/03/30/122382.abstract
AB The objective of our study was to develop novel techniques for investigations of cell motility, and to assess whether the electric field of the therapeutic spinal cord stimulation system used in vivo contributes to the migration of human mesenchymal stem cells (hMSCs) in vitro.We have investigated electrotaxis of bone marrow-derived MSCs using pulsed electric field (PEF) in range 16-80 mV/mm and frequency 130 Hz and 240 Hz. The PEF-related dynamics of the cell surface glycosylation was evaluated using six plant lectins.PEF at physiological levels (10mV/mm; 130 Hz) did not influence cellular motility in vitro, what may correspond to the maintenance of the transplanted cells at the lesion site in vivo. Increase of the PEF intensity and frequency above physiological levels resulted in the increase in the cellular migration rate in vitro. PEF elevated above physiological intensity and frequency (40-80 mV/mm; 240 Hz), but not at physiological levels, resulted in changes of the cell surface glycosylation.We find the described approach as convenient for investigations and for the in vitro modeling of the cellular systems intended for the regenerative cell transplantations in vivo. Probing cell surface glycomes may provide valuable biomarkers to assess competence of transplanted cells.