RT Journal Article
SR Electronic
T1 RNA-seq: primary cells, cell lines and heat stress
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 013979
DO 10.1101/013979
A1 Carl J. Schmidt
A1 Elizabeth M. Pritchett
A1 Liang Sun
A1 Richard V.N. Davis
A1 Allen Hubbard
A1 Kalmia E. Kniel
A1 Sarah M. Markland
A1 Qing Wang
A1 Chris Ashwell
A1 Michael Persia
A1 Max F. Rothschild
A1 Susan J. Lamont
YR 2015
UL http://biorxiv.org/content/early/2015/01/19/013979.abstract
AB Transcriptome analysis by RNA-seq has emerged as a high-throughput, cost-effective means to evaluate the expression pattern of genes in organisms. Unlike other methods, such as microarrays or quantitative PCR, RNA-seq is a target free method that permits analysis of essentially any RNA that can be amplified from a cell or tissue. At its most basic, RNA-seq can determine individual gene expression levels by counting the number of times a particular transcript was found in the sequence data. Transcript levels can be compared across multiple samples to identify differentially expressed genes and infer differences in biological states between the samples. We have used this approach to examine gene expression patterns in chicken and human cells, with particular interest in determining response to heat stress.