RT Journal Article SR Electronic T1 Dynamics of dynein at microtubule plus-ends and the cortex during the division of the C. elegans zygote JF bioRxiv FD Cold Spring Harbor Laboratory SP 118604 DO 10.1101/118604 A1 Ruddi Rodriguez Garcia A1 Laurent Chesneau A1 Sylvain Pastezeur A1 Julien Roul A1 Marc Tramier A1 Jacques Pécréaux YR 2017 UL http://biorxiv.org/content/early/2017/03/21/118604.abstract AB During asymmetric cell divisions, cortical dyneins generate forces essential to position the spindle after polarity cues, prescribing daughter cells fate. In nematode zygote, cortical dynein pulls on microtubules transiently, raising the question of its targeting and dynamics. Tracking and fluorescence correlation spectroscopy revealed that in the cytoplasm, dynein spots displayed directed motions toward the cortex, localized at microtubule plus-ends through EBP-2/EB but are not actively transported. Surprisingly CLIP-1/CLIP170 is not involved. ebp-2(0) slightly reduced spindle rocking, thus most cortical forces remain suggesting a redundant mechanism.At the cortex, to relate dynein residency and forces generating, we tracked dynein and found two dynamically distinct populations. One of them would correspond to force generating events. Our experiments also indicated that an asymmetric (polarized) dynein-microbutule on-rate, causes the force imbalance positioning the spindle. GPR-1/2 increases the overall dynein density but also residency time (pulling processivity), although this latter was not polarized.