PT  - JOURNAL ARTICLE
AU  - Nikhil Biyani
AU  - Ricardo D. Righetto
AU  - Robert McLeod
AU  - Daniel Caujolle-Bert
AU  - Daniel Castano-Diez
AU  - Kenneth N. Goldie
AU  - Henning Stahlberg
TI  - Focus: The interface between data collection and data processing in cryo-EM
AID  - 10.1101/105452
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 105452
4099  - http://biorxiv.org/content/early/2017/03/17/105452.short
4100  - http://biorxiv.org/content/early/2017/03/17/105452.full
AB  - We present a new software package called Focus that interfaces cryo-transmission electron microscopy (cryo-EM) data collection with computer image processing. Focus creates a user-friendly environment to import and manage data recorded by direct electron detectors and perform elemental image processing tasks in a high-throughput manner while new data is being acquired at the microscope. It provides the functionality required to remotely monitor the progress of data collection and data processing, which is essential now that automation in cryo-EM allows a steady flow of images of single particles, two-dimensional crystals, or electron tomography data to be recorded in overnight sessions. The rapid detection of any errors that may occur greatly increases the productivity of recording sessions at the electron microscope.Abbreviationscryo-EMCryo-electron microscopyDEDDirect electron detector2DTwo dimensional3DThree dimensionalCTFContrast transfer functionFFTFast Fourier transformGPUGraphical processing unitCPUCentral processing unitsMRCMRC file formatSNRSignal-to-noise ratioGUIGraphical user interface