TY - JOUR T1 - Focus: The interface between data collection and data processing in cryo-EM JF - bioRxiv DO - 10.1101/105452 SP - 105452 AU - Nikhil Biyani AU - Ricardo D. Righetto AU - Robert McLeod AU - Daniel Caujolle-Bert AU - Daniel Castano-Diez AU - Kenneth N. Goldie AU - Henning Stahlberg Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/03/17/105452.abstract N2 - We present a new software package called Focus that interfaces cryo-transmission electron microscopy (cryo-EM) data collection with computer image processing. Focus creates a user-friendly environment to import and manage data recorded by direct electron detectors and perform elemental image processing tasks in a high-throughput manner while new data is being acquired at the microscope. It provides the functionality required to remotely monitor the progress of data collection and data processing, which is essential now that automation in cryo-EM allows a steady flow of images of single particles, two-dimensional crystals, or electron tomography data to be recorded in overnight sessions. The rapid detection of any errors that may occur greatly increases the productivity of recording sessions at the electron microscope.Abbreviationscryo-EMCryo-electron microscopyDEDDirect electron detector2DTwo dimensional3DThree dimensionalCTFContrast transfer functionFFTFast Fourier transformGPUGraphical processing unitCPUCentral processing unitsMRCMRC file formatSNRSignal-to-noise ratioGUIGraphical user interface ER -