PT  - JOURNAL ARTICLE
AU  - John Easton
AU  - Veronica Gonzalez Pena
AU  - Donald Yergeau
AU  - Xiaotu Ma
AU  - Charles Gawad
TI  - Genome-wide Segregation of Single Nucleotide and Structural Variants into Single Cancer Cells
AID  - 10.1101/117663
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 117663
4099  - http://biorxiv.org/content/early/2017/03/16/117663.short
4100  - http://biorxiv.org/content/early/2017/03/16/117663.full
AB  - We present a new approach for determining comprehensive variant profiles of single cells using a microfluidic amplicon-based strategy. This method can be used to reconstruct the clonal architecture and mutational history of a malignancy using all classes and sizes of single nucleotide and structural variants, providing insights into the temporal changes in mutational classes and processes that led to the development of a cancer. Using this approach, we interrogated single cells from a patient with leukemia, determining that processes producing structural variation preceded single nucleotides changes in the development of that malignancy.