TY - JOUR T1 - Genome-wide Segregation of Single Nucleotide and Structural Variants into Single Cancer Cells JF - bioRxiv DO - 10.1101/117663 SP - 117663 AU - John Easton AU - Veronica Gonzalez Pena AU - Donald Yergeau AU - Xiaotu Ma AU - Charles Gawad Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/03/16/117663.abstract N2 - We present a new approach for determining comprehensive variant profiles of single cells using a microfluidic amplicon-based strategy. This method can be used to reconstruct the clonal architecture and mutational history of a malignancy using all classes and sizes of single nucleotide and structural variants, providing insights into the temporal changes in mutational classes and processes that led to the development of a cancer. Using this approach, we interrogated single cells from a patient with leukemia, determining that processes producing structural variation preceded single nucleotides changes in the development of that malignancy. ER -