RT Journal Article
SR Electronic
T1 Rapid genome recoding by iterative recombineering of synthetic DNA
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 115493
DO 10.1101/115493
A1 Yu Heng Lau
A1 Finn Stirling
A1 James Kuo
A1 Michiel A.P. Karrenbelt
A1 Yujia A. Chan
A1 Adam Riesselman
A1 Connor A. Horton
A1 Elena Schaefer
A1 David Lips
A1 Matthew T. Weinstock
A1 Daniel G. Gibson
A1 Jeffrey C. Way
A1 Pamela A. Silver
YR 2017
UL http://biorxiv.org/content/early/2017/03/09/115493.abstract
AB Genome recoding will provide a deeper understanding of genetics and transform biotechnology. We bypass the reliance of previous genome recoding methods on site-specific enzymes and demonstrate a rapid recombineering based strategy for writing genomes by Stepwise Integration of Rolling Circle Amplified Segments (SIRCAS). We installed the largest number of codon substitutions in a single organism yet published, creating a strain of Salmonella typhimurium with 1557 leucine codon changes across 200 kb of the genome.