@article {Lau115493, author = {Yu Heng Lau and Finn Stirling and James Kuo and Michiel A.P. Karrenbelt and Yujia A. Chan and Adam Riesselman and Connor A. Horton and Elena Schaefer and David Lips and Matthew T. Weinstock and Daniel G. Gibson and Jeffrey C. Way and Pamela A. Silver}, title = {Rapid genome recoding by iterative recombineering of synthetic DNA}, elocation-id = {115493}, year = {2017}, doi = {10.1101/115493}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Genome recoding will provide a deeper understanding of genetics and transform biotechnology. We bypass the reliance of previous genome recoding methods on site-specific enzymes and demonstrate a rapid recombineering based strategy for writing genomes by Stepwise Integration of Rolling Circle Amplified Segments (SIRCAS). We installed the largest number of codon substitutions in a single organism yet published, creating a strain of Salmonella typhimurium with 1557 leucine codon changes across 200 kb of the genome.}, URL = {https://www.biorxiv.org/content/early/2017/03/09/115493}, eprint = {https://www.biorxiv.org/content/early/2017/03/09/115493.full.pdf}, journal = {bioRxiv} }