RT Journal Article
SR Electronic
T1 CRISPR/Cas9 screening using unique molecular identifiers
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 114355
DO 10.1101/114355
A1 Bernhard Schmierer
A1 Sandeep K. Botla
A1 Jilin Zhang
A1 Mikko Turunen
A1 Teemu Kivioja
A1 Jussi Taipale
YR 2017
UL http://biorxiv.org/content/early/2017/03/06/114355.abstract
AB Loss of function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, random sequence labels (RSLs) were incorporated into the guide-library. RSLs function as internal replicates for robust and reproducible hit calling, and act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing (MPLT) and true dropout screening.