PT - JOURNAL ARTICLE AU - Bernhard Schmierer AU - Sandeep K. Botla AU - Jilin Zhang AU - Mikko Turunen AU - Teemu Kivioja AU - Jussi Taipale TI - CRISPR/Cas9 screening using unique molecular identifiers AID - 10.1101/114355 DP - 2017 Jan 01 TA - bioRxiv PG - 114355 4099 - http://biorxiv.org/content/early/2017/03/06/114355.short 4100 - http://biorxiv.org/content/early/2017/03/06/114355.full AB - Loss of function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, random sequence labels (RSLs) were incorporated into the guide-library. RSLs function as internal replicates for robust and reproducible hit calling, and act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing (MPLT) and true dropout screening.