RT Journal Article
SR Electronic
T1 Quantifying Cellular Capacity to Identify Gene Expression Designs With Reduced Burden
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 013110
DO 10.1101/013110
A1 Francesca Ceroni
A1 Rhys Algar
A1 Guy-Bart Stan
A1 Tom Ellis
YR 2014
UL http://biorxiv.org/content/early/2014/12/22/013110.abstract
AB Heterologous gene expression can be a significant burden to cells, consuming resources and causing decreased growth and stability. We describe here an in vivo monitor that tracks E. coli capacity changes in real-time and can be used to assay the burden synthetic constructs and their parts impose. By measuring capacity, construct designs with reduced burden can be identifiedand shown to predictably outperform less efficient designs, despite having equivalent expression outputs.