PT  - JOURNAL ARTICLE
AU  - Marlon Stoeckius
AU  - Christoph Hafemeister
AU  - William Stephenson
AU  - Brian Houck-Loomis
AU  - Harold Swerdlow
AU  - Rahul Satija
AU  - Peter Smibert
TI  - Large-scale simultaneous measurement of epitopes and transcriptomes in single cells
AID  - 10.1101/113068
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 113068
4099  - http://biorxiv.org/content/early/2017/03/03/113068.short
4100  - http://biorxiv.org/content/early/2017/03/03/113068.full
AB  - Recent high-throughput single-cell sequencing approaches have been transformative for understanding complex cell populations, but are unable to provide additional phenotypic information, such as protein levels of well-established cell-surface markers. Using oligonucleotide-labeled antibodies, we integrate measurements of cellular proteins and transcriptomes into an efficient, sequencing-based readout of single cells. This method is compatible with existing single-cell sequencing approaches and will readily scale as the throughput of these methods increase.