TY - JOUR T1 - CAR T Cells Secreting IL18 Augment Antitumor Immunity and Increase T Cell Proliferation and Costimulation JF - bioRxiv DO - 10.1101/111260 SP - 111260 AU - Biliang Hu AU - Jiangtao Ren AU - Yanping Luo AU - Brian Keith AU - Regina M. Young AU - John Scholler AU - Yangbing Zhao AU - Carl H. June Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/02/27/111260.abstract N2 - Interleukin 18 (IL18) is known to induce the expression of interferon-γ (IFNG), but its effects on T cell proliferation and costimulation are not completely understood. In this study, we demonstrate that ectopic expression of IL18 in CART cells caused significant T cell proliferation in vitro and in vivo, and enhanced antitumor effects in xenograft models. Moreover, IL18 mediated T cell expansion required neither tumor antigen nor CAR expression, and produced severe GVHD in NSG mice. Furthermore, recombinant IL18 costimulated IFNG secretion and proliferation of anti-CD3 beads treated T cells. Interestingly, IL18 costimulation could expand purified CD4 T cells, but not CD8 T cells. However, CD8 T cells proliferated greater than CD4 T cells in magnitude within bulk T cells, suggesting CD4 help effect was involved. Using CRISPR/Cas9 gene editing, we confirmed that IL18-driven expansion was both TCR and IL18 receptor (IL18R) dependent. Importantly, we demonstrated that TCR-deficient, IL18-expressing CD19 CART cells exhibited remarkable proliferation and persistent antitumor activity against CD19-expressing tumor cells in vivo, without eliciting any detectable GVHD symptom. Finally, we describe APACHE T cells, a novel strategy for coupling IL18 expression in CART cells to antigen stimulation, thereby limiting potential toxicity associated with persistent IL18 production. In sum, our study supports human IL18 as a T cell costimulatory cytokine for fueling CART therapy.TCRT Cell ReceptorCARChimeric Antigen ReceptorIL18-CARTIL18 producing CARTCD19-IL18 CARTIL18 producing CD19 CARTSS1-IL18 CARTIL18 producing SS1 CARTTILTumor Infiltrating LymphocytesGVHDGraft-Versus-Host DiseaseCRISPRClustered regularly interspaced short palindromic repeatsCas9CRISPR associated protein 9IL18RKnockoutKOIL18 ReceptorNFATNuclear Factor of Activated T-cellsaAPCartificial Antigen Presenting Cells ER -