@article {Hu111260, author = {Biliang Hu and Jiangtao Ren and Yanping Luo and Brian Keith and Regina M. Young and John Scholler and Yangbing Zhao and Carl H. June}, title = {CAR T Cells Secreting IL18 Augment Antitumor Immunity and Increase T Cell Proliferation and Costimulation}, elocation-id = {111260}, year = {2017}, doi = {10.1101/111260}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Interleukin 18 (IL18) is known to induce the expression of interferon-γ (IFNG), but its effects on T cell proliferation and costimulation are not completely understood. In this study, we demonstrate that ectopic expression of IL18 in CART cells caused significant T cell proliferation in vitro and in vivo, and enhanced antitumor effects in xenograft models. Moreover, IL18 mediated T cell expansion required neither tumor antigen nor CAR expression, and produced severe GVHD in NSG mice. Furthermore, recombinant IL18 costimulated IFNG secretion and proliferation of anti-CD3 beads treated T cells. Interestingly, IL18 costimulation could expand purified CD4 T cells, but not CD8 T cells. However, CD8 T cells proliferated greater than CD4 T cells in magnitude within bulk T cells, suggesting CD4 help effect was involved. Using CRISPR/Cas9 gene editing, we confirmed that IL18-driven expansion was both TCR and IL18 receptor (IL18R) dependent. Importantly, we demonstrated that TCR-deficient, IL18-expressing CD19 CART cells exhibited remarkable proliferation and persistent antitumor activity against CD19-expressing tumor cells in vivo, without eliciting any detectable GVHD symptom. Finally, we describe APACHE T cells, a novel strategy for coupling IL18 expression in CART cells to antigen stimulation, thereby limiting potential toxicity associated with persistent IL18 production. In sum, our study supports human IL18 as a T cell costimulatory cytokine for fueling CART therapy.TCRT Cell ReceptorCARChimeric Antigen ReceptorIL18-CARTIL18 producing CARTCD19-IL18 CARTIL18 producing CD19 CARTSS1-IL18 CARTIL18 producing SS1 CARTTILTumor Infiltrating LymphocytesGVHDGraft-Versus-Host DiseaseCRISPRClustered regularly interspaced short palindromic repeatsCas9CRISPR associated protein 9IL18RKnockoutKOIL18 ReceptorNFATNuclear Factor of Activated T-cellsaAPCartificial Antigen Presenting Cells}, URL = {https://www.biorxiv.org/content/early/2017/02/27/111260}, eprint = {https://www.biorxiv.org/content/early/2017/02/27/111260.full.pdf}, journal = {bioRxiv} }