TY - JOUR T1 - An alternative STAT signaling pathway acts in antiviral immunity in <em>Caenorhabditis elegans</em> JF - bioRxiv DO - 10.1101/110940 SP - 110940 AU - Mélanie Tanguy AU - Louise Véron AU - Przemyslaw Stempor AU - Julie Ahringer AU - Peter Sarkies AU - Eric A. Miska Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/02/25/110940.abstract N2 - Across metazoans, innate immunity is vital in defending organisms against viral infection. In mammals, antiviral innate immunity is orchestrated by interferon signaling, activating the STAT transcription factors downstream of the JAK kinases to induce expression of antiviral effector genes. In the nematode C. elegans, which lacks the interferon system, the major antiviral response so far described is RNA interference but whether additional gene expression responses are employed is not known. Here we show that, despite the absence of both interferon and JAK, the C. elegans STAT homologue STA-1 orchestrates antiviral immunity. Intriguingly, mutants lacking STA-1 show increased resistance to antiviral infection. Using gene expression analysis and chromatin immunoprecipitation we show that, in contrast to the mammalian pathway, STA-1 acts as a transcriptional repressor. Thus STA-1 might act to suppress a constitutive antiviral response in the absence of infection. Using a reverse genetic screen we identify the SID-3 as a kinase upstream of STA-1 in the response to infection. Together, our work identifies a novel STAT regulatory cascade controlling its activity in antiviral resistance, illustrating the complex evolutionary trajectory displayed by innate immune signaling pathways across metazoan organisms. ER -