RT Journal Article SR Electronic T1 More Signal, Less Noise: Single-primer Adenylation Method (SAM) for the Efficient Preparation of DNA for Transposon Sequencing (TnSeq) JF bioRxiv FD Cold Spring Harbor Laboratory SP 012732 DO 10.1101/012732 A1 S Willcocks A1 R Stabler A1 B Wren YR 2014 UL http://biorxiv.org/content/early/2014/12/13/012732.abstract AB Transposon Sequencing (TnSeq) is a cutting-edge tool that allows quantitative, genome-wide genetic analysis of a given population in an experimental condition. TnSeq utilises next generation sequencing that is designed to perform optimally when all of the input material is of interest, therefore TnSeq is inefficient since only a small portion of nucleic acid fragments contain the transposon target site for primer annealing. We describe an innovative modification to standard transposon library preparation protocols that provides a significant improvement to the quality of sequencing data recovered.