PT - JOURNAL ARTICLE AU - S Willcocks AU - R Stabler AU - B Wren TI - More Signal, Less Noise: Single-primer Adenylation Method (SAM) for the Efficient Preparation of DNA for Transposon Sequencing (TnSeq) AID - 10.1101/012732 DP - 2014 Jan 01 TA - bioRxiv PG - 012732 4099 - http://biorxiv.org/content/early/2014/12/13/012732.short 4100 - http://biorxiv.org/content/early/2014/12/13/012732.full AB - Transposon Sequencing (TnSeq) is a cutting-edge tool that allows quantitative, genome-wide genetic analysis of a given population in an experimental condition. TnSeq utilises next generation sequencing that is designed to perform optimally when all of the input material is of interest, therefore TnSeq is inefficient since only a small portion of nucleic acid fragments contain the transposon target site for primer annealing. We describe an innovative modification to standard transposon library preparation protocols that provides a significant improvement to the quality of sequencing data recovered.