PT - JOURNAL ARTICLE AU - Tomoe Takano, M.Sc. AU - Miyako Funabiki, M.D. AU - Sagiri Taguchi, M.D., Ph.D. AU - Fumie Saji, Ph.D. AU - Namiko Amano, B.Sc. AU - Kate Young Louise, B.Sc. AU - Yoshitaka Nakamura, M.D. TI - Can the location of a trophectoderm biopsy contribute to human blastocyst development ? AID - 10.1101/109298 DP - 2017 Jan 01 TA - bioRxiv PG - 109298 4099 - http://biorxiv.org/content/early/2017/02/20/109298.short 4100 - http://biorxiv.org/content/early/2017/02/20/109298.full AB - The influence of the location of a trophectoderm biopsy in human blastocysts on the development of those blastocysts has not yet been investigated. In our prospective study (n=92), our multivariate logistic regression analysis indicated that blastocoel development was influenced by the location of the trophectoderm biopsy (p=0.049) and by the type of human blastocyst used (fresh or thawed) (p=0.037), regardless of the patient’s age (p=0.507) and the number of days for the human blastocyst in the pretrophectoderm biopsy (p=0.239). Therefore, when a trophectoderm biopsy is close to the inner cell mass (ICM) in human blastocysts, it improves the progress of blastocoel development.Clinical evidence suggests that the progress of blastocoel development is a predictor of clinical outcomes after single blastocyst transfer. Therefore, when the trophectoderm biopsy is done from near the ICM, improvement of clinical outcomes after single blastocyst transfer may be expected.