RT Journal Article
SR Electronic
T1 Genome sequencing reveals Zika virus diversity and spread in the Americas
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 109348
DO 10.1101/109348
A1 H.C. Metsky
A1 C.B. Matranga
A1 S. Wohl
A1 S.F. Schaner
A1 C.A. Freije
A1 S. M. Winnicki
A1 K. West
A1 J. Qu
A1 M.L. Baniecki
A1 A. Gladden-Young
A1 A.E. Lin
A1 C.H. Tomkins-Tinch
A1 D.J. Park
A1 C.Y. Luo
A1 K.G. Barnes
A1 B. Chak
A1 G. Barbosa-Lima
A1 E. Delatorre
A1 Y.R. Vieira
A1 L.M. Paul
A1 A.L. Tan
A1 M.C. Porcelli
A1 C. Vasquez
A1 A.C. Cannons
A1 M.R. Cone
A1 K.N. Hogan
A1 E.W. Kopp IV
A1 J.J. Anzinger
A1 K.F. Garcia
A1 L.A. Parham
A1 R.M. Gélvez Ramírez
A1 M.C. Miranda Montoya
A1 D.P. Rojas
A1 C.M. Brown
A1 S. Hennigan
A1 B. Sabina
A1 S. Scotland
A1 K. Gangavarapu
A1 N.D. Grubaugh
A1 G. Oliveira
A1 R. Robles-Sikisaka
A1 A. Rambaut
A1 L. Gehrke
A1 S. Smole
A1 M.E. Halloran
A1 L.A. Villar Centeno
A1 S. Mattar
A1 I. Lorenzana
A1 J. Cerbino-Neto
A1 W. Degrave
A1 P.T. Bozza
A1 A. Gnirke
A1 K.G. Andersen
A1 S. Isern
A1 S. Michael
A1 F. Bozza
A1 T.M.L. Souza
A1 I. Bosch
A1 N.L. Yozwiak
A1 B.L. MacInnis
A1 P.C. Sabeti
YR 2017
UL http://biorxiv.org/content/early/2017/02/18/109348.abstract
AB Despite great attention given to the recent Zika virus (ZIKV) epidemic in the Americas, much remains unknown about its epidemiology and evolution, in part due to a lack of genomic data. We applied multiple sequencing approaches to generate 100 ZIKV genomes from clinical and mosquito samples from 10 countries and territories, greatly expanding the observed viral genetic diversity from this outbreak. We analyzed the timing and patterns of introductions into distinct geographic regions, confirming phylogenetic evidence for the origin and rapid expansion of the outbreak in Brazil1, and for multiple introductions from Brazil into Honduras, Colombia, Puerto Rico, other Caribbean islands, and the continental US. We find that ZIKV circulated undetected in many regions of the Americas for up to a year before the first locally transmitted cases were confirmed, highlighting the challenge of effective surveillance for this virus. We further characterize genetic variation across the outbreak to identify mutations with possible functional implications for ZIKV biology and pathogenesis.