PT  - JOURNAL ARTICLE
AU  - Christian M. K. Sieber
AU  - Alexander J. Probst
AU  - Allison Sharrar
AU  - Brian C. Thomas
AU  - Matthias Hess
AU  - Susannah G. Tringe
AU  - Jillian F. Banfield
TI  - Recovery of genomes from metagenomes via a dereplication, aggregation, and scoring strategy
AID  - 10.1101/107789
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 107789
4099  - http://biorxiv.org/content/early/2017/02/11/107789.short
4100  - http://biorxiv.org/content/early/2017/02/11/107789.full
AB  - Microbial communities are critical to ecosystem function. A key objective of metagenomic studies is to analyse organism-specific metabolic pathways and reconstruct community interaction networks. This requires accurate assignment of assembled genome fragments to genomes. Existing binning methods often fail to reconstruct a reasonable number of genomes and report many bins of low quality and completeness. Furthermore, the performance of existing algorithms varies between samples and biotopes. Here, we present a dereplication, aggregation and scoring strategy, DAS Tool, that combines the strengths of a flexible set of established binning algorithms. DAS Tool applied to a constructed community generated more accurate bins than any automated method. Further, when applied to environmental and host-associated samples of different complexity, DAS Tool recovered substantially more near-complete genomes, including novel lineages, than any single binning method alone. The ability to reconstruct many near-complete genomes from metagenomics data will greatly advance genome-centric analyses of ecosystems.