TY - JOUR T1 - A high-resolution mRNA expression time course of embryonic development in zebrafish JF - bioRxiv DO - 10.1101/107631 SP - 107631 AU - Richard J. White AU - John E. Collins AU - Ian M. Sealy AU - Neha Wali AU - Christopher M. Dooley AU - Zsofia Digby AU - Derek L. Stemple AU - Daniel N. Murphy AU - Thibaut Hourlier AU - Anja Füllgrabe AU - Matthew P. Davis AU - Anton J. Enright AU - Elisabeth M. Busch-Nentwich Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/02/10/107631.abstract N2 - We have produced an mRNA expression time course of early zebrafish development across 18 time points from 1-cell to 5 days post-fertilisation sampling individual and pools of embryos. Using poly(A) pulldown stranded RNA-seq as well as a 3′ end transcript counting method we characterise the temporal expression profiles of 23,642 genes. We identify temporal and functional transcript co-variance that associates 5,024 unnamed genes with distinct developmental time points. Specifically, a class of over 100 previously uncharacterised zinc finger domain containing genes, all located on the long arm of chromosome 4, is expressed in a sharp peak during zygotic genome activation. The data reveal complex and widespread differential use of exons, previously unidentified 3′ ends across development, new primary microRNA transcripts and the temporal divergence of gene paralogues generated in the teleost genome duplication. To make this dataset a useful baseline reference, the data are accessible to browse and download at Expression Atlas and Ensembl. ER -