RT Journal Article SR Electronic T1 Focus: The interface between data collection and data processing in cryo-EM JF bioRxiv FD Cold Spring Harbor Laboratory SP 105452 DO 10.1101/105452 A1 Nikhil Biyani A1 Ricardo D. Righetto A1 Robert McLeod A1 Daniel Caujolle-Bert A1 Daniel Castano-Diez A1 Kenneth N. Goldie A1 Henning Stahlberg YR 2017 UL http://biorxiv.org/content/early/2017/02/03/105452.abstract AB We present a new software package called Focus that interfaces cryo-transmission electron microscopy (cryo-EM) data collection with computer image processing. Focus creates a userfriendly environment to import and manage data recorded by direct electron detectors and perform elemental image processing tasks in a high-throughput manner, while new data is being acquired at the microscope. It provides the functionality required to remotely monitor the progress of data collection and data processing, which is essential now that automation in cryo-EM allows a steady flow of images of single particles, two-dimensional crystals, or electron tomography data to be recorded in overnight sessions. The rapid detection of any errors that may occur greatly increases the productivity of recording sessions at the electron microscope.Cryo-EMCryo-electron microscopyDEDDirect electron detector2DTwo dimensional3DThree dimensionalCTFContrast transfer functionFFTFast Fourier transformGPUGraphical processing unitCPUCentral processing unitsMRCMRC file formatSNRSignal-to-noise ratioGUIGraphical user interface