TY - JOUR T1 - Morphogen signalling patterns calcium waves in the Drosophila wing disc JF - bioRxiv DO - 10.1101/104745 SP - 104745 AU - Qinfeng Wu AU - Pavel Brodskiy AU - Cody Narciso AU - Megan Levis AU - Ninfamaria Arredondo-Walsh AU - Jeremiah J. Zartman Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/02/01/104745.abstract N2 - Intercellular Ca2+ waves (ICWs) are multicellular, coordinated oscillations of Ca2+ that transverse tissues and are functionally implicated in development, regeneration, and diseases such as cancer. However, the extent that spontaneous ICWs are a regulated phenomenon controlled by cell-cell communication networks is unclear. Here we report that ICWs exhibit spatiotemporal patterns at the organ-level using a new image analysis algorithm to quantify ICW dynamics. ICWs in the Drosophila wing disc require a specific phospholipase C, Plc21C. Further, we demonstrate that the morphogen signalling pathway, Hedgehog, modulates ICW frequency uniformly in the tissue through two distinct routes and is required for non-uniform spatial patterning of ICW amplitudes. Thus, the dynamics of spontaneous ICWs are regulated by morphogenetic signalling through two orthogonal mechanisms—frequency and amplitude—and provide an organ-scale communication system for the developing wing disc. ER -