PT  - JOURNAL ARTICLE
AU  - Marcela de Souza Santos
AU  - Dor Salomon
AU  - Kim Orth
TI  - T3SS effector VopL inhibits the host ROS response, promoting the intracellular survival of <em>Vibrio parahaemolyticus</em>
AID  - 10.1101/104315
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 104315
4099  - http://biorxiv.org/content/early/2017/01/30/104315.short
4100  - http://biorxiv.org/content/early/2017/01/30/104315.full
AB  - The production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (NADPH) oxidase complex is an important mechanism for control of invading pathogens. Herein, we show that the gastrointestinal pathogen Vibrio parahaemolyticus counteracts reactive oxygen species (ROS) production using the Type III Secretion System 2 (T3SS2) effector VopL. In the absence of VopL, intracellular V. parahaemolyticus undergo ROS-dependent filamentation, with concurrent limited growth. During infection, VopL assembles actin into non-functional filaments resulting in a dysfunctional actin cytoskeleton that can no longer mediate the assembly of the NADPH oxidase at the cell membrane, thereby limiting ROS production. This is the first example of how a T3SS2 effector contributes to the intracellular survival of V. parahaemolyticus to support the establishment of a protective intracellular replicative niche.