TY - JOUR T1 - Mechanical activation of epithelial Na<sup>+</sup> channel relies on an interdependent activity of the extracellular matrix and extracellular <em>N</em>-glycans of αENaC JF - bioRxiv DO - 10.1101/102756 SP - 102756 AU - Fenja Knoepp AU - Zoe Ashley AU - Daniel Barth AU - Marina Kazantseva AU - Pawel P. Szczesniak AU - Wolfgang G. Clauss AU - Mike Althaus AU - Diego Alvarez de la Rosa AU - Martin Fronius Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/01/24/102756.abstract N2 - Mechanotransduction describes how cells perceive their mechanical environment and mechanosensitive ion channels are important for this process. ENaC (epithelial Na+ channel)/DEG (degenerin) proteins form mechanosensitive ion channels and it is hypothesized their interaction with the extracellular matrix (ECM) via ‘tethers’ is required for mechanotransduction. Channels formed by vertebrate α, β and γ ENaC proteins are activated by shear force (SF) and mediate electrolyte/fluid-homeostasis and blood pressure regulation. Here, we report an interdependent activity of ENaC and the ECM that mediates SF effects in murine arteries and heterologously expressed channels. Furthermore, replacement of conserved extracellular N-glycosylated asparagines of αENaC decreased the SF response indicating that the attached N-glycans provide a connection to the ECM. Insertion of N-glycosylation sites into a channel subunit, innately lacking these motifs, increased its SF response. These experiments confirm an interdependent channel/ECM activity of mechanosensitive ENaC channel and highlight the role of channel N-glycans as new constituents for the translation of mechanical force into cellular signals. ER -