PT  - JOURNAL ARTICLE
AU  - Sunghyeok Ye
AU  - Taegeun Bae
AU  - Kyoungmi Kim
AU  - Omer Habib
AU  - Seung Hwan Lee
AU  - Yoon Young Kim
AU  - Kang-In Lee
AU  - Seokjoong Kim
AU  - Jin-Soo Kim
TI  - DNA-dependent RNA cleavage by the Natronobacterium gregoryi Argonaute
AID  - 10.1101/101923
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 101923
4099  - http://biorxiv.org/content/early/2017/01/20/101923.short
4100  - http://biorxiv.org/content/early/2017/01/20/101923.full
AB  - We show here that, unlike most other prokaryotic Argonaute (Ago) proteins, which are DNA-guided endonucleases, the Natronobacterium gregoryi-derived Ago (NgAgo) can function as a DNA-guided endoribonuclease, cleaving RNA, rather than DNA, in a targeted manner. The NgAgo protein, in complex with 5’-hydroxylated or 5’-phosphrylated oligodeoxyribonucleotides (ODNs) of variable lengths, split RNA targets into two or more fragments in vitro, suggesting its physiological role in bacteria and demonstrating a potential for degrading RNA molecules such as mRNA or lncRNA in eukaryotic cells in a targeted manner.