RT Journal Article
SR Electronic
T1 An Improved Crystal Violet Assay for Biofilm Quantification in 96-Well Microtitre Plate
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 100214
DO 10.1101/100214
A1 Sudhir K. Shukla
A1 T. Subba Rao
YR 2017
UL http://biorxiv.org/content/early/2017/01/13/100214.abstract
AB Microplates are essential tools for biofilm research since it allows high throughput screening of biofilm forming strains or in the assay of anti-biofilm drugs. However, 96 well microtitre plate based assays share the issue of “edge effect”. The primary cause of the “edge effect” phenomenon is evaporation. As edge effect causes a significant increase in plate rejection rate by introducing experimental error, we improvised the classical crystal violet assay to reduce water loss from the peripheral wells. The improvised method showed a significant reduction in edge effect and minimised error in crystal violet assay