TY - JOUR T1 - An Improved Crystal Violet Assay for Biofilm Quantification in 96-Well Microtitre Plate JF - bioRxiv DO - 10.1101/100214 SP - 100214 AU - Sudhir K. Shukla AU - T. Subba Rao Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/01/13/100214.abstract N2 - Microplates are essential tools for biofilm research since it allows high throughput screening of biofilm forming strains or in the assay of anti-biofilm drugs. However, 96 well microtitre plate based assays share the issue of “edge effect”. The primary cause of the “edge effect” phenomenon is evaporation. As edge effect causes a significant increase in plate rejection rate by introducing experimental error, we improvised the classical crystal violet assay to reduce water loss from the peripheral wells. The improvised method showed a significant reduction in edge effect and minimised error in crystal violet assay ER -