@article {Shukla100214, author = {Sudhir K. Shukla and T. Subba Rao}, title = {An Improved Crystal Violet Assay for Biofilm Quantification in 96-Well Microtitre Plate}, elocation-id = {100214}, year = {2017}, doi = {10.1101/100214}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Microplates are essential tools for biofilm research since it allows high throughput screening of biofilm forming strains or in the assay of anti-biofilm drugs. However, 96 well microtitre plate based assays share the issue of {\textquotedblleft}edge effect{\textquotedblright}. The primary cause of the {\textquotedblleft}edge effect{\textquotedblright} phenomenon is evaporation. As edge effect causes a significant increase in plate rejection rate by introducing experimental error, we improvised the classical crystal violet assay to reduce water loss from the peripheral wells. The improvised method showed a significant reduction in edge effect and minimised error in crystal violet assay}, URL = {https://www.biorxiv.org/content/early/2017/01/13/100214}, eprint = {https://www.biorxiv.org/content/early/2017/01/13/100214.full.pdf}, journal = {bioRxiv} }