RT Journal Article
SR Electronic
T1 Large-scale rewiring in a yeast hybrid
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 100263
DO 10.1101/100263
A1 Rebecca H. Herbst
A1 Dana Bar-Zvi
A1 Sharon Reikhav
A1 Ilya Soifer
A1 Michal Breker
A1 Ghil Jona
A1 Eyal Shimoni
A1 Maya Schuldiner
A1 Avraham Levy
A1 Naama Barkai
YR 2017
UL http://biorxiv.org/content/early/2017/01/13/100263.abstract
AB The merging of genomes in inter-specific hybrids can result in novel phenotypes, including increased growth rate and biomass yield, a phenomenon known as heterosis. We describe a budding yeast hybrid that grows faster than its parents under different environments. Phenotypically, the hybrid progresses more rapidly through cell cycle checkpoints, relieves the repression of respiration in fast growing conditions, does not slow down its growth when presented with ethanol stress, and shows increasing signs of DNA damage. A systematic genetic screen identified hundreds of alleles affecting hybrid growth whose identity vastly differed between the hybrid and its parent and between growth conditions. This large-scale rewiring of allele effects suggests that despite showing clear heterosis, the hybrid is perturbed in multiple regulatory processes. We discuss the possibility that incompatibilities contribute to hybrid vigor by perturbing safeguard mechanisms that limit growth in the parental background.