RT Journal Article SR Electronic T1 CRISPR-RT: A web service for designing CRISPR-C2c2 crRNA with improved target specificity JF bioRxiv FD Cold Spring Harbor Laboratory SP 099895 DO 10.1101/099895 A1 Houxiang Zhu A1 Emily Richmond A1 Chun Liang YR 2017 UL http://biorxiv.org/content/early/2017/01/12/099895.abstract AB CRISPR-Cas systems have been successfully applied in genome editing. Recently, the CRISPR-C2c2 system has been reported as a tool for RNA editing. Here we describe CRISPR-RT (CRISPR RNA-Targeting), the first web service to help biologists design the crRNA with improved target specificity for the CRISPR-C2c2 system. CRISPR-RT allows users to set up a wide range of parameters, making it highly flexible for current and future research in CRISPR-based RNA editing. CRISPR-RT covers major model organisms and can be easily extended to cover other species. CRISPR-RT will empower researchers in RNA editing. It is available at http://bioinfolab.miamioh.edu/CRISPR-RT.