TY - JOUR T1 - On the (im)possibility to reconstruct plasmids from whole genome short-read sequencing data JF - bioRxiv DO - 10.1101/086744 SP - 086744 AU - Sergio Arredondo-Alonso AU - Willem van Schaik AU - Rob J. Willems AU - Anita C. Schürch Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/01/11/086744.abstract N2 - Plasmids are autonomous extra-chromosomal elements in bacterial cells that can carry genes that are important for bacterial survival. To benchmark algorithms for automated plasmid sequence reconstruction from short read sequencing data, we selected 42 publicly available complete bacterial genome sequences with associated sequencing reads from 12 genera, containing 148 plasmids. We predicted plasmids from WGS with four different programs (PlasmidSPAdes, Recycler, cBar and PlasmidFinder) and compared the outcome to the reference sequences.PlasmidSPAdes reconstructs plasmids based on coverage differences in the assembly graph. It reconstructed most of the reference plasmids (recall = 0.82) with approximately a quarter of the predicted sequences corresponding to false positives (precision = 0.76). A total of 83.1 % of the reconstructions from genomes with multiple plasmids were merged and manual steps were necessary to separate individual plasmid sequences. Recycler searches the assembly graph for sub-graphs corresponding to circular sequences. It correctly predicted small plasmids but failed with long plasmids (recall = 0.12, precision = 0.28). cBar, which applies pentamer frequency composition analysis to detect plasmid-derived contigs, showed an overall recall and precision of 0.77 and 0.63. However, cBar only categorizes contigs as plasmid-derived and does not bin the different plasmids correctly within a bacterial isolate. PlasmidFinder, which searches for matches in a replicon database, had the highest precision (1.0) but was restricted by the contents of its database and the contig length obtained from de novo assembly (recall = 0.33). We conclude that without long read information, plasmid reconstruction from WGS remains challenging and error-prone. ER -