PT  - JOURNAL ARTICLE
AU  - Sha Joe Zhu
AU  - Jacob Almagro-Garcia
AU  - Gil McVean
TI  - Deconvoluting multiple infections in <em>Plasmodium falciparum</em> from high throughput sequencing data
AID  - 10.1101/099499
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 099499
4099  - http://biorxiv.org/content/early/2017/01/10/099499.short
4100  - http://biorxiv.org/content/early/2017/01/10/099499.full
AB  - Motivation: The presence of multiple infecting strains of the malarial parasite Plasmodium falciparum affects key phenotypic traits, including drug resistance and risk of severe disease. Advances in protocols and sequencing technology have made it possible to obtain high-coverage genome-wide sequencing data from blood samples and blood spots taken in the field. However, analysing and interpreting such data is challenging because of the high rate of multiple infections present.Results: We have developed a statistical method and implementation for deconvoluting multiple genome sequences present in an individual with mixed infections. The software package DEploid uses haplotype structure within a reference panel of clonal isolates as a prior for haplotypes present in a given sample. It estimates the number of strains, their relative proportions and the haplotypes presented in a sample, allowing researchers to study multiple infection in malaria with an unprecedented level of detail.Results: The open source implementation DEploid is freely available at https://github.com/mcveanlab/dEploid under the conditions of the GPLv3 license. An R version is available at https://github.com/mcveanlab/DEploid-r.