RT Journal Article
SR Electronic
T1 Resolving microbial microdiversity with high accuracy, full length 16S rRNA Illumina sequencing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 010967
DO 10.1101/010967
A1 Catherine M. Burke
A1 Aaron E. Darling
YR 2014
UL http://biorxiv.org/content/early/2014/11/06/010967.abstract
AB We describe a method for sequencing full-length 16S rRNA gene amplicons using the high throughput Illumina MiSeq platform. The resulting sequences have about 100-fold higher accuracy than standard Illumina reads and are chimera filtered using information from a single molecule dual tagging scheme that boosts the signal available for chimera detection. We demonstrate that the data provides fine scale phylogenetic resolution not available from Illumina amplicon methods targeting smaller variable regions of the 16S rRNA gene.