PT - JOURNAL ARTICLE AU - Catherine M. Burke AU - Aaron E. Darling TI - Resolving microbial microdiversity with high accuracy, full length 16S rRNA Illumina sequencing AID - 10.1101/010967 DP - 2014 Jan 01 TA - bioRxiv PG - 010967 4099 - http://biorxiv.org/content/early/2014/11/06/010967.short 4100 - http://biorxiv.org/content/early/2014/11/06/010967.full AB - We describe a method for sequencing full-length 16S rRNA gene amplicons using the high throughput Illumina MiSeq platform. The resulting sequences have about 100-fold higher accuracy than standard Illumina reads and are chimera filtered using information from a single molecule dual tagging scheme that boosts the signal available for chimera detection. We demonstrate that the data provides fine scale phylogenetic resolution not available from Illumina amplicon methods targeting smaller variable regions of the 16S rRNA gene.