RT Journal Article
SR Electronic
T1 CENP-A associated lncRNAs influence chromosome segregation in human cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 097956
DO 10.1101/097956
A1 Delphine Quénet
A1 David Sturgill
A1 Marin Olson
A1 Yamini Dalal
YR 2017
UL http://biorxiv.org/content/early/2017/01/03/097956.abstract
AB Transcription occurs ubiquitously throughout non-coding parts of the genome, including at repetitive α-satellite DNA elements which comprise the majority of human centromeres. The function of temporally regulated centromeric transcription, and transcripts, is consequently a topic of intense investigation. In this study, we use high throughput approaches to identify and describe lncRNAs associated with the centromere specific histone variant CENP-A that arise from the transcription of specific centromeres at early G1, which we then show are physically associated with centromeres, and which are functionally necessary for accurate chromosome segregation. Targeted depletion of one such centromeric RNA, which originates from a single centromere, is sufficient to increase the frequency of chromosome segregation defects. These data support the emerging paradigm of the necessity of centromere-specific lncRNAs in the integrity of faithful chromosome segregation.