RT Journal Article
SR Electronic
T1 Enhanced Transcriptome Maps from Multiple Mouse Tissues Reveal Evolutionary Constraint in Gene Expression for Thousands of Genes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 010884
DO 10.1101/010884
A1 Dmitri D. Pervouchine
A1 Sarah Djebali
A1 Alessandra Breschi
A1 Carrie A. Davis
A1 Pablo Prieto Barja
A1 Alex Dobin
A1 Andrea Tanzer
A1 Julien Lagarde
A1 Chris Zaleski
A1 Lei-Hoon See
A1 Meagan Fastuca
A1 Jorg Drenkow
A1 Huaien Wang
A1 Giovanni Bussotti
A1 Baikang Pei
A1 Suganthi Balasubramanian
A1 Jean Monlong
A1 Arif Harmanci
A1 Mark Gerstein
A1 Michael A. Beer
A1 Cedric Notredame
A1 Roderic Guigó
A1 Thomas R. Gingeras
YR 2014
UL http://biorxiv.org/content/early/2014/10/30/010884.abstract
AB We characterized by RNA-seq the transcriptional profiles of a large and heterogeneous collection of mouse tissues, augmenting the mouse transcriptome with thousands of novel transcript candidates. Comparison with transcriptome profiles obtained in human cell lines reveals substantial conservation of transcriptional programs, and uncovers a distinct class of genes with levels of expression across cell types and species, that have been constrained early in vertebrate evolution. This core set of genes capture a substantial and constant fraction of the transcriptional output of mammalian cells, and participates in basic functional and structural housekeeping processes common to all cell types. Perturbation of these constrained genes is associated with significant phenotypes including embryonic lethality and cancer. Evolutionary constraint in gene expression levels is not reflected in the conservation of the genomic sequences, but is associated with strong and conserved epigenetic marking, as well as to a characteristic post-transcriptional regulatory program in which sub-cellular localization and alternative splicing play comparatively large roles.