RT Journal Article
SR Electronic
T1 Integrating discovery-driven proteomics and selected reaction monitoring to develop a non-invasive assay for geoduck reproductive maturation
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 094615
DO 10.1101/094615
A1 Emma B. Timmins-Schiffman
A1 Grace A. Crandall
A1 Brent Vadopalas
A1 Michael E. Riffle
A1 Brook L. Nunn
A1 Steven B. Roberts
YR 2016
UL http://biorxiv.org/content/early/2016/12/28/094615.abstract
AB Geoduck clams (Panopea generosa) are an increasingly important fishery and aquaculture product along the eastern Pacific coast from Baja California, Mexico to Alaska. These long-lived clams are highly fecund, though sustainable hatchery production of genetically diverse larvae is hindered by the lack of sexual dimorphism, resulting in asynchronous spawning of broodstock, unequal sex ratios, and low numbers of breeders. Development of assays of gonad physiology would indicate sex, maturation stage, as well as be used to assess status of natural populations. Proteomic profiles were determined for three reproductive maturation stages in both male and female clams using data dependent acquisition (DDA) of gonad proteins. Gonad proteomes became increasingly divergent between males and females as maturation progressed. The DDA data was used to develop targets analyzed with selected reaction monitoring (SRM) in gonad tissue as well as hemolymph. The SRM assay yielded a suite of indicator peptides that can be used as an efficient assay to determine geoduck gonad maturation status. Application of SRM in hemolymph samples demonstrates this procedure could effectively be used to assess reproductive status in marine mollusks in a non-lethal manner.