RT Journal Article
SR Electronic
T1 HILAQ: A Novel Strategy for Newly Synthesized Protein Quantification
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 096925
DO 10.1101/096925
A1 Yuanhui Ma
A1 Daniel B. McClatchy
A1 Salim Barkallah
A1 William W. Wood
A1 John R. Yates III
YR 2016
UL http://biorxiv.org/content/early/2016/12/28/096925.abstract
AB Here we describe a new strategy, HILAQ (Heavy Isotope Labeled Azidohomoalanine Quantification), to rapidly quantify the molecular vulnerability profile to oxytosis. HILAQ quantified more than 1,900 Newly Synthesized Proteins (NSP) after 1h pulse labeling in HEK293T cell line. Compared with the previous published QuaNCAT protocol, the HILAQ strategy simplifies newly synthesized proteome analysis and achieves higher sensitivity.