PT  - JOURNAL ARTICLE
AU  - Yuanhui Ma
AU  - Daniel B. McClatchy
AU  - Salim Barkallah
AU  - William W. Wood
AU  - John R. Yates III
TI  - HILAQ: A Novel Strategy for Newly Synthesized Protein Quantification
AID  - 10.1101/096925
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 096925
4099  - http://biorxiv.org/content/early/2016/12/28/096925.short
4100  - http://biorxiv.org/content/early/2016/12/28/096925.full
AB  - Here we describe a new strategy, HILAQ (Heavy Isotope Labeled Azidohomoalanine Quantification), to rapidly quantify the molecular vulnerability profile to oxytosis. HILAQ quantified more than 1,900 Newly Synthesized Proteins (NSP) after 1h pulse labeling in HEK293T cell line. Compared with the previous published QuaNCAT protocol, the HILAQ strategy simplifies newly synthesized proteome analysis and achieves higher sensitivity.